Relation of virus particle counts to the hemagglutinating activity of influenza virus suspensions measured by the HA pattern test and by use of the photometric HCU method
Identifieur interne : 002B91 ( Main/Exploration ); précédent : 002B90; suivant : 002B92Relation of virus particle counts to the hemagglutinating activity of influenza virus suspensions measured by the HA pattern test and by use of the photometric HCU method
Auteurs : U. Desselberger [Allemagne]Source :
- Archives of Virology [ 0304-8608 ] ; 1975-12-01.
English descriptors
- Teeft :
- Different batches, Different days, Different influenza virus strains, Different strains, Excellent agreement, Hemagglutinating, Hemagglutinating activity, Hemagglutinating subunits, Hemagglutinating viruses, Influenza, Influenza virus, Influenza virus particles, Influenza virus strains, Influenza virus suspensions, Other investigators, Other strains, Particle counts, Pattern test, Photometric, Photometric methods, Significant differences, Standard deviation, Such differences, Titration, Virus, Virus particles, Virus suspension, Virus suspensions.
Abstract
Summary: The ratios of the number of virus particles (n.v.p.) to the hemagglutinating activity were determined for a series of influenza A and B strains using both the photometric HCU method and the HA pattern test. The logarithms of the ratios of n.v.p./HCU ranged from 7.06 to 7.54 and the logarithms of the ratios of n.v.p./HA from 6.87 to 7.56. While the n.v.p./HA ratios of different strains showed significant differences in a number of cases, such differences were not found for the n.v.p./HCU ratios with the only exception of the strain B/Hong Kong/8/73 differing uniformly from the other strains tested. Since the standard deviation of HCU values ranged from 0.006 to 0.039 log units and that of HA titers from 0.076 to 0.272 log units, it was concluded that the differences of n.v.p./HA ratios found were due to the range of variance of HA tests and did not reflect strain specific differences. The degree of purity of virus suspensions was found not to influence measurably the log n.v.p./HCU values.
Url:
DOI: 10.1007/BF01318246
Affiliations:
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Desselberger</name><affiliation wicri:level="3"><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Virology, Medizinische Hochschule Hannover, Hannover</wicri:regionArea><placeName><region type="land" nuts="2">Basse-Saxe</region><settlement type="city">Hanovre</settlement></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Archives of Virology</title><title level="j" type="abbrev">Archives of Virology</title><idno type="ISSN">0304-8608</idno><idno type="eISSN">1432-8798</idno><imprint><publisher>Springer-Verlag</publisher><pubPlace>Vienna</pubPlace><date type="published" when="1975-12-01">1975-12-01</date><biblScope unit="volume">49</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="365">365</biblScope><biblScope unit="page" to="372">372</biblScope></imprint><idno type="ISSN">0304-8608</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0304-8608</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Different batches</term><term>Different days</term><term>Different influenza virus strains</term><term>Different strains</term><term>Excellent agreement</term><term>Hemagglutinating</term><term>Hemagglutinating activity</term><term>Hemagglutinating subunits</term><term>Hemagglutinating viruses</term><term>Influenza</term><term>Influenza virus</term><term>Influenza virus particles</term><term>Influenza virus strains</term><term>Influenza virus suspensions</term><term>Other investigators</term><term>Other strains</term><term>Particle counts</term><term>Pattern test</term><term>Photometric</term><term>Photometric methods</term><term>Significant differences</term><term>Standard deviation</term><term>Such differences</term><term>Titration</term><term>Virus</term><term>Virus particles</term><term>Virus suspension</term><term>Virus suspensions</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Summary: The ratios of the number of virus particles (n.v.p.) to the hemagglutinating activity were determined for a series of influenza A and B strains using both the photometric HCU method and the HA pattern test. The logarithms of the ratios of n.v.p./HCU ranged from 7.06 to 7.54 and the logarithms of the ratios of n.v.p./HA from 6.87 to 7.56. While the n.v.p./HA ratios of different strains showed significant differences in a number of cases, such differences were not found for the n.v.p./HCU ratios with the only exception of the strain B/Hong Kong/8/73 differing uniformly from the other strains tested. Since the standard deviation of HCU values ranged from 0.006 to 0.039 log units and that of HA titers from 0.076 to 0.272 log units, it was concluded that the differences of n.v.p./HA ratios found were due to the range of variance of HA tests and did not reflect strain specific differences. The degree of purity of virus suspensions was found not to influence measurably the log n.v.p./HCU values.</div></front></TEI><affiliations><list><country><li>Allemagne</li></country><region><li>Basse-Saxe</li></region><settlement><li>Hanovre</li></settlement></list><tree><country name="Allemagne"><region name="Basse-Saxe"><name sortKey="Desselberger, U" sort="Desselberger, U" uniqKey="Desselberger U" first="U." last="Desselberger">U. Desselberger</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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